SrasV1, Main, Exploration, bibRecord, 004011

A Severe Acute Respiratory Syndrome Coronavirus That Lacks the E Gene Is Attenuated In Vitro and In Vivo▿

Identifieur interne : 004011 ( Main/Exploration ); précédent : 004010; suivant : 004012

A Severe Acute Respiratory Syndrome Coronavirus That Lacks the E Gene Is Attenuated In Vitro and In Vivo▿

Auteurs : Marta L. Dediego ; Enrique Álvarez ; Fernando Almazán ; María Teresa Rejas ; Elaine Lamirande ; Anjeanette Roberts ; Wun-Ju Shieh ; Sherif R. Zaki ; Kanta Subbarao ; Luis Enjuanes

Source :

Journal of Virology [ 0022-538X ] ; 2006.

RBID : PMC:1797558

Descripteurs français

KwdFr :
- Animaux, Appareil respiratoire (virologie), Cellules Caco-2, Cellules Vero, Cricetinae, Délétion de gène, Humains, Protéines virales structurales (génétique), Réplication virale, Syndrome respiratoire aigu sévère (virologie), Virus du SRAS (physiologie).
MESH :
- génétique : Protéines virales structurales.
- physiologie : Virus du SRAS.
- virologie : Appareil respiratoire, Syndrome respiratoire aigu sévère.
- Animaux, Cellules Caco-2, Cellules Vero, Cricetinae, Délétion de gène, Humains, Réplication virale.

English descriptors

KwdEn :
- Animals, Caco-2 Cells, Chlorocebus aethiops, Cricetinae, Gene Deletion, Humans, Respiratory System (virology), SARS Virus (physiology), Severe Acute Respiratory Syndrome (virology), Vero Cells, Viral Structural Proteins (genetics), Virus Replication.
MESH :
- chemical , genetics : Viral Structural Proteins.
- physiology : SARS Virus.
- virology : Respiratory System, Severe Acute Respiratory Syndrome.
- Animals, Caco-2 Cells, Chlorocebus aethiops, Cricetinae, Gene Deletion, Humans, Vero Cells, Virus Replication.

Abstract

A deletion mutant of severe acute respiratory syndrome coronavirus (SARS-CoV) has been engineered by deleting the structural E gene in an infectious cDNA clone that was constructed as a bacterial artificial chromosome (BAC). The recombinant virus lacking the E gene (rSARS-CoV-ΔE) was rescued in Vero E6 cells. The recovered deletion mutant grew in Vero E6, Huh-7, and CaCo-2 cells to titers 20-, 200-, and 200-fold lower than the recombinant wild-type virus, respectively, indicating that although the E protein has an effect on growth, it is not essential for virus replication. No differences in virion stability under a wide range of pH and temperature were detected between the deletion mutant and recombinant wild-type viruses. Although both viruses showed the same morphology by electron microscopy, the process of morphogenesis seemed to be less efficient with the defective virus than with the recombinant wild-type one. The rSARS-CoV-ΔE virus replicated to titers 100- to 1,000-fold lower than the recombinant wild-type virus in the upper and lower respiratory tract of hamsters, and the lower viral load was accompanied by less inflammation in the lungs of hamsters infected with rSARS-CoV-ΔE virus than with the recombinant wild-type virus. Therefore, the SARS-CoV that lacks the E gene is attenuated in hamsters, might be a safer research tool, and may be a good candidate for the development of a live attenuated SARS-CoV vaccine.

Url:

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1797558

DOI: 10.1128/JVI.01467-06
PubMed: 17108030
PubMed Central: 1797558

Affiliations:

Le document en format XML

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<series><title level="j">Journal of Virology</title>
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<term>Gene Deletion</term>
<term>Humans</term>
<term>Respiratory System (virology)</term>
<term>SARS Virus (physiology)</term>
<term>Severe Acute Respiratory Syndrome (virology)</term>
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<term>Cellules Vero</term>
<term>Cricetinae</term>
<term>Délétion de gène</term>
<term>Humains</term>
<term>Protéines virales structurales (génétique)</term>
<term>Réplication virale</term>
<term>Syndrome respiratoire aigu sévère (virologie)</term>
<term>Virus du SRAS (physiologie)</term>
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<keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>SARS Virus</term>
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<term>Severe Acute Respiratory Syndrome</term>
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<term>Cellules Vero</term>
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<front><div type="abstract" xml:lang="en"><p>A deletion mutant of severe acute respiratory syndrome coronavirus (SARS-CoV) has been engineered by deleting the structural E gene in an infectious cDNA clone that was constructed as a bacterial artificial chromosome (BAC). The recombinant virus lacking the E gene (rSARS-CoV-ΔE) was rescued in Vero E6 cells. The recovered deletion mutant grew in Vero E6, Huh-7, and CaCo-2 cells to titers 20-, 200-, and 200-fold lower than the recombinant wild-type virus, respectively, indicating that although the E protein has an effect on growth, it is not essential for virus replication. No differences in virion stability under a wide range of pH and temperature were detected between the deletion mutant and recombinant wild-type viruses. Although both viruses showed the same morphology by electron microscopy, the process of morphogenesis seemed to be less efficient with the defective virus than with the recombinant wild-type one. The rSARS-CoV-ΔE virus replicated to titers 100- to 1,000-fold lower than the recombinant wild-type virus in the upper and lower respiratory tract of hamsters, and the lower viral load was accompanied by less inflammation in the lungs of hamsters infected with rSARS-CoV-ΔE virus than with the recombinant wild-type virus. Therefore, the SARS-CoV that lacks the E gene is attenuated in hamsters, might be a safer research tool, and may be a good candidate for the development of a live attenuated SARS-CoV vaccine.</p>
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<affiliations><list></list>
<tree><noCountry><name sortKey="Almazan, Fernando" sort="Almazan, Fernando" uniqKey="Almazan F" first="Fernando" last="Almazán">Fernando Almazán</name>
<name sortKey="Alvarez, Enrique" sort="Alvarez, Enrique" uniqKey="Alvarez E" first="Enrique" last="Álvarez">Enrique Álvarez</name>
<name sortKey="Dediego, Marta L" sort="Dediego, Marta L" uniqKey="Dediego M" first="Marta L." last="Dediego">Marta L. Dediego</name>
<name sortKey="Enjuanes, Luis" sort="Enjuanes, Luis" uniqKey="Enjuanes L" first="Luis" last="Enjuanes">Luis Enjuanes</name>
<name sortKey="Lamirande, Elaine" sort="Lamirande, Elaine" uniqKey="Lamirande E" first="Elaine" last="Lamirande">Elaine Lamirande</name>
<name sortKey="Rejas, Maria Teresa" sort="Rejas, Maria Teresa" uniqKey="Rejas M" first="María Teresa" last="Rejas">María Teresa Rejas</name>
<name sortKey="Roberts, Anjeanette" sort="Roberts, Anjeanette" uniqKey="Roberts A" first="Anjeanette" last="Roberts">Anjeanette Roberts</name>
<name sortKey="Shieh, Wun Ju" sort="Shieh, Wun Ju" uniqKey="Shieh W" first="Wun-Ju" last="Shieh">Wun-Ju Shieh</name>
<name sortKey="Subbarao, Kanta" sort="Subbarao, Kanta" uniqKey="Subbarao K" first="Kanta" last="Subbarao">Kanta Subbarao</name>
<name sortKey="Zaki, Sherif R" sort="Zaki, Sherif R" uniqKey="Zaki S" first="Sherif R." last="Zaki">Sherif R. Zaki</name>
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A Severe Acute Respiratory Syndrome Coronavirus That Lacks the E Gene Is Attenuated In Vitro and In Vivo▿

A Severe Acute Respiratory Syndrome Coronavirus That Lacks the E Gene Is Attenuated In Vitro and In Vivo▿

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

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